منابع مشابه
A database for globin gene expression data
We describe a prototype database of sequence alignments and experimental results for the P-like globin gene cluster of mammals. This data repository is intended to help the international community of globingene biologists to plan experiments, to design models and, ultimately, to understand regulation of those genes. Moreover, the approaches and software developed for th.is project will be appli...
متن کاملExpression of a cloned Lepore globin gene.
Lepore globin is synthesized in markedly diminished amounts (approximately 10% to 15% of normal beta-globin) in human erythroid cells. To study the molecular mechanisms responsible for the diminished biosynthesis of Lepore globin, the Lepore-Boston gene was cloned from a charon phage DNA library and expressed in HeLa cells. Northern blotting and S1 nuclease analyses indicated that the Lepore ge...
متن کاملDevelopmental regulation of globin gene expression.
We have used the globin family of genes in chicken to study developmental regulation of gene expression, both at the level of individual interaction of trans-acting factors with local promoters and enhancers, and at the level of chromatin structure. Regulation of all members of the alpha- and beta-globin clusters is affected by the erythroid regulatory factor GATA-1. Separate mechanisms exist f...
متن کاملFine Tuning of Globin Gene Expression by DNA Methylation
Expression patterns in the globin gene cluster are subject to developmental regulation in vivo. While the gamma(A) and gamma(G) genes are expressed in fetal liver, both are silenced in adult erythrocytes. In order to decipher the role of DNA methylation in this process, we generated a YAC transgenic mouse system that allowed us to control gamma(A) methylation during development. DNA methylation...
متن کاملGlobin gene expression in erythroid human fetal liver cells.
We measured steady-state levels of the human globin mRNAs in liver samples from several mid-gestational fetuses. RNA from the epsilon, gamma, beta, zeta, theta, and alpha globin genes were present in fetal liver samples isolated from 10-25-wk embryos. The abundance of all human globin mRNAs declined in older fetuses, presumably because of a gradual reduction in the proportion of erythroid precu...
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ژورنال
عنوان ژورنال: Molecular Therapy - Methods & Clinical Development
سال: 2019
ISSN: 2329-0501
DOI: 10.1016/j.omtm.2018.12.004